Differences between laminar flow hoods and a biological safety cabinets are typically measured according to the type of protection provided to the user, the laboratory and the environment.
Laminar flow cabinets are available in two types, horizontal and vertical. Each have their own advantages, depending on the application being used. Biological safety cabinets are classified by NSF as Class I, Class II, Class III and others with sub-divisions based on size and level of protection.
When is horizontal the best choice?
Choose a horizonal laminar flow cabinet when using small utensils and equipment that will not cause airflow turbulence. Horizontal laminar cabinets are often used in clinical or pharmaceutical applications, or whenever a sterile, particulate-free situation is required. The air from a horizontal flow hood goes back into the environment, making it unusable for biohazardous substances.
When is vertical the best choice?
Choose a vertical laminar flow cabinet when using larger equipment as the work space is taller and deeper. The air from a vertical flow hood goes back into the environment untreated so only non-hazardous materials should be handled in the work zone.
When to choose a biological safety cabinet:
Biological safety cabinets must be used when protection of the user and the environment is required. There are several options of class and varying levels of protection available in BSC’s. Class I Biological safety cabinets are designed solely for operator protection. Class II and Class III protect the product or specimen, the user, and the environment from contamination. Class II cabinets have open access to the work zone, while Class III BSC’s, often referred to as glove boxes, provide a barrier between the user and the work area. A Class III biosafety cabinet is crucial for working with any biosafety level 4 agents, or other dangerous materials, such as aerosols of pathogens or toxins.
